Catalytic pKa Attenuation in a Hydrolytic Metalloenzyme by Genetic Code Expansion
Manser BP, Deliz Liang A*. 2026, Biochemistry: 10.1021/acs.biochem.5c00768
Dataset
Genetic incorporation of diverse non-canonical amino acids for histidine substitution
Natter Perdiguero A⧧, Fischer S⧧, Lischke AR, Manser BP, Deliz Liang A*. 2026, JACS: 10.1021/jacs.5c19599
BioRxiv 2025: 10.1101/2025.11.04.686677
Dataset
and addgene deposit is awaiting release by addgene
Hydrophobic tuning with non-canonical amino acids in a copper metalloenzyme
Fischer S⧧, Natter Perdiguero A⧧, Lau K, Deliz Liang A*. 2025, BioRxiv: 10.1101/2025.02.09.636911
Dataset and
Plasmids
Practical Approaches to Genetic Code Expansion with Aminoacyl-tRNA Synthetase/tRNA Pairs
Natter Perdiguero A, Deliz Liang A*. CHIMIA 2024, 78, 22. 10.2533/chimia.2024.22
HaloTag Engineering for Enhanced Fluorogenicity and Kinetics with a Styrylpyridium Dye
Miró-Vinyals C, Stein A, Fischer S, Ward TR, Deliz Liang A*. ChemBioChem. 2021, 10.1002/cbic.202100424.
Engineering a Metathesis-Catalyzing Artificial Metalloenzyme Based on HaloTag
Fischer S, Ward TR*, Deliz Liang A*. ACS Catalysis. 2021, 11, 6343.
Ultrahigh-Throughput Screening of an Artificial Metalloenzyme using Double Emulsions
Vallapurackal J, Stucki A, Deliz Liang A, Klehr J, Dittrich PS, Ward TR. Angew. Chem., Int. Ed. 2022. 10.1002/anie.202207328
Incorporation of metal-chelating unnatural amino acids into halotag for allylic deamination
Stein A, Deliz Liang A, Sahin R, Ward TR. J Organomet. Chem. 2022, 962, 122272.
Artificial Metalloenzymes Based on the Biotin-Streptavidin Technology: Enzymatic Cascades and Directed Evolution
Liang AD, Serrano-Plana J, Petterson RL, Ward TR. Acc. Chem. Res. 2019, 52, 585.
Genetically Encoded Protein Phosphorylation in Mammalian Cells
Beranek V, Reinkemeier CD, Zhang MS, Liang AD, Kym G, Chin JW. Cell Chem. Biol. 2018, 25, 1067.
Biosynthesis and Genetic Encoding of Phosphothreonine through Parallel Selections and Deep Sequencing
Zhang SM, Brunner SF, Huguenin-Dezot N, Liang AD, Schmied WH, Rogerson DT, and Chin JW, Nat. Methods. 2017, 14, 729.
Single Turnover Reveals Oxygenated Intermediates in Toluene/o-Xylene Monooxygenase in the Presence of the Native Redox Partners
Liang AD and Lippard SJ, J. Am. Chem. Soc. 2015, 137, 10520.
Coupling Oxygen Consumption with Hydrocarbon Oxidation in Bacterial Multicomponent Monooxygenases
Wang W, Liang AD, and Lippard SJ, Acc. Chem. Res. 2015, 48, 2632.
Solid-Phase Synthesis Provides a Modular, Lysine-Based Platform for Fluorescent Discrimination of Nitroxyl and Biological Thiols
Loas A, Radford RJ, Liang AD, and Lippard SJ, Chem. Sci. 2015, 6, 4131.
Component Interactions and Electron Transfer in Toluene/o-Xylene Monooxygenase
Liang AD and Lippard SJ, Biochemistry, 2014, 53, 7368.
A Flexible Glutamine Regulates Catalytic Function of Toluene/o-Xylene Monooxygenase
Liang AD, Wrobel AT, and Lippard SJ, Biochemistry, 2014, 53, 3585.
A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl
Wrobel WT, Johnstone TC, Liang AD, Lippard SJ, Rivera-Fuentes P, J. Am. Chem. Soc. 2014, 136, 4697.
Tyrosyl Radicals in Dehaloperoxidase: How Nature Deals with Evolving an Oxygen-Binding Globin to a Biologically Relevant Peroxidase
Dumarieh R, D’Antonio J, Deliz-Liang A, Smirnova T, Svistunenko DA, Ghiladi RA, J. Biol. Chem. 2013, 288, 33470.